Additionally, in another study, which administered the Trier Social Stress Test (in which stress is caused to participants and their salivary cortisol levels thereby raised), the increase of salivary cortisol in a group who took the test while listening to relaxing music was significantly suppressed compared to that of a control group. In a previous study, conducted in patients who had just undergone the stressful experience of learning in detail about the procedures involved in surgery that they would undergo the next day, salivary cortisol was significantly reduced among a group who listened to music for one hour, compared to a group who did not. In the endocrine system, cortisol and chromogranin A have been used as indicators of stress. It is known that listening to music affects the endocrine system and autonomic nervous system. In this study, we measured salivary biomarkers of stress (cortisol, chromogranin A, and oxytocin), which can be collected noninvasively, as indices of stress relief. Accordingly, we examined the effect of such music compared to 440 Hz music. Various effects have been ascribed to the solfeggio frequency, but none of these have any scientific basis. In general, this specific type of music using a scale including 528 Hz is called solfeggio frequency music. We refer to music that is tuned and composed in this way as 528 Hz music. However, setting the reference tone to 444 Hz means that 528 Hz is included in the musical scale. In this musical scale, there is no 528 Hz note. Usually, the reference tone of tuning is 440 Hz, and this is the international standard frequency (we refer to this as 440 Hz music). Music of the aforementioned frequency (528 Hz) has lately attracted attention as вЂњhealingвЂќ music. Therefore, in the present study, we examined the stress reduction effect of music with a frequency of 528 Hz by measuring its influence on the endocrine system and autonomic nervous system. Furthermore, others report that listening to high-frequency music increases the activity of the parasympathetic nervous system and reduces stress, in comparison to listening to low-frequency music. This result suggests that music including high-frequency sound stimulates dopamine synthesis and suppresses sympathetic nervous system activity. In a study in which rats were exposed to musical stimuli of different frequencies, the ratsвЂ™ blood pressure decreased depending on the frequency notably, this change was observed to a greater extent for music in the frequency range of 16 kHz compared to 4 kHz frequency. However, few studies have investigated how differences in the frequency of music affect the human body. Previous studies that have examined emotional arousal in response to listening to music have suggested that music arouses different emotions depending on its characteristics, such as melody, rhythm, and dynamism. Research has investigated many aspects of this phenomenon, and it is recognized that several constituent elements of music are involved in this stress reduction effect. It is well known that listening to music reduces stress. These results suggest that the influence of music on the autonomic nervous system and endocrine system varies depending on the frequency of the music, and furthermore, that 528 Hz music has an especially strong stress-reducing effect, even following only five minutes of exposure. Tension-anxiety and Total Mood Disturbance scores were significantly reduced after exposure to 528 Hz music, while there was no significant difference following 440 Hz music. The ratio of low frequency to high frequency autonomic nervous system activity significantly decreased after exposure to both types of music, and the coefficient of variation of R-R intervals also significantly decreased, but only after exposure to 528 Hz music. However, no significant change was observed in any salivary biomarkers in the 440 Hz condition.

In the 528 Hz condition, mean levels of cortisol significantly decreased, chromogranin A tended to decrease, and oxytocin significantly increased after music exposure. The Profile of Mood State, 2nd edition, was also administered as a subjective indicator of stress. We measured salivary biomarkers of stress (cortisol, chromogranin A, and oxytocin) before and after exposure to music, and continuously recorded the activity of the autonomic nervous system. Nine healthy participants (one man and eight women, aged 26 - 37 years) listened to 528 Hz and standard 440 Hz music on separate days. This study examined the stress-reducing effect on the endocrine system and the autonomic nervous system of music with a frequency of 528 Hz, which has recently attracted attention as a вЂњhealingвЂќ type of music.

Solfeggio frequencies chart tones